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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in many tumors, which results in chromosome instability, and so may lead to tumorigenesis. This protein was discovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two added splice variants had been noted but detailed nucleotide sequence data is just not but obtainable.
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Blocking peptides are peptides that bind particularly to your concentrate on antibody and block antibody binding. These peptide usually consists of the epitope acknowledged because of the antibody.
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This manual outlines even further doable optimization of mobile and immunoassay parameters to be sure the very best success are...
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This guideline outlines even further feasible optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which lead towards the temporal coordination of every mitotic occasion. Cyclin E kinds a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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